Proteomics and Functional Genomics (01:694:412)
This course is intended for undergraduate Molecular Biology and Biochemistry (MBB) majors or those with a strong background in the Biological Sciences. It may be used to fulfill the elective requirements of Biological Sciences or MBB majors.
Prerequisites: Molecular Biology and Biochemistry 01:694:407 and Genetics 01:447:380
This course is a survey of modern techniques of protein biochemistry, bioinformatics, proteomics, and functional genomics. It will include a discussion of basic concepts of protein structure and function, protein characterization and purification, enzyme kinetics, nmr and x-ray crystallography, mass spectrometry, and various techniques of functional and structural genomics.
1 Course introduction and overview of genomics & proteomics
2 New strategies and technologies for DNA sequencing
3 DNA sequencing – current technologies & applications
4 Applications of new DNA sequencing technologies (con’d)
5 Amino acids and proteins
6 Conformational properties of proteins
7 Blast and dynamic programming algorithm
8 Sequence-based bioinformatics
9 Analytical and preparative protein chemistry
10 Preparative protein chemistry (cont’d)
11 Protein domains and folds
Take-home Exam I handed out
12 Structural Bioinformatics
13 Protein folding in vitro
Take-home Exam I due
14 Protein folding in vivo
15 Protein NMR and structure analysis
16 Protein X-ray crystallography
17 Protein Mass Spectrometry and Proteomics
18 Cryo Electron Microscopy. Homology Modeling
19 Protein-protein interactions I
20 Protein-protein interactions II
21 Enzyme kinetics
22 Enzyme kinetics / Protein dynamics
Take-home Exam II handed out
23 Student Presentations
24 Student Presentations
Take-home Exam II due
25 Student Presentations
26 Student Presentations
27 Student Presentations
28 Student Presentations
Take-home Exam III
Course URL: All course materials will be posted on Sakai
Course satisfies Learning Goals
1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research in the areas of proteomics, functional genomics, and/or bioinformatics. They will learn the theory and practice of key methods needed for postgraduate studies in these fields. .
2. Students should demonstrate the ability to work independently to find and evaluate information about specific biological systems or problems.
Exams, Assignments, and Grading Policy
Take-home Exams I & II will each count for 30% of your grade. The Student Presentation counts for 25% of your grade. Take-home Exam III counts for 15% of your grade. We also take into account class participation, especially in the Student Presentation part of the course. There will be no final exam.
Course Materials: Readings provided by instructors.
This course is by special permission only, with priority given to MBB majors. The majority of the class will be juniors and seniors. To obtain a special permission number or to get on the waiting list if you are not an MBB major, please use the following link:Wait List Sign Up for Spring 2018 Courses If you have any questions, please contact the Division of Life Sciences - Office of Undergraduate Instruction at 848-445-2075 or visit our office at Nelson Biological Laboratories B112, Busch Campus.
Prof. Gaetano Montelione
Prof. Stephen Anderson
** All information is subject to change at the discretion of the course coordinator.