01:694:408 MOLECULAR BIOLOGY AND BIOCHEMISTRY
This course is intended for Life Science majors or those with a strong background in the Biological Sciences.
This course may be used to fulfill the elective requirements of the Biological Sciences major.
Prerequisite for 01:694:408 is 01:694:407 (preferred), or 01:694:301, 11:115:301, or 11:115:403. Credit not given for both 01:694:408 and 01:146:478 or 11:126:481.
Molecular Biology and Biochemistry 01: 694:407-408 is offered as the primary lecture courses for undergraduate students in the Molecular Biology and Biochemistry major, as well as for non-majors who wish to enroll in a contemporary, comprehensive two-semester course in these rapidly developing disciplines. Biochemistry 11:115:511-512 mainly serves graduate students in Graduate Programs other than Molecular Biosciences. Topics in the 408/512 course include recombinant DNA approaches, DNA replication, repair and recombination, mobile genetic elements, transcription and gene regulation, RNA processing and splicing, and translation.
A Sakai site will be set up at the beginning of the semester
Tentative Lecture Schedule
|#||Day||Topic||Instructor||Chapter in Watson et al 6th ed.|
|2||Fri.||Bacterial DNA Replication I||Ch8: 4:66-69|
|3||Tues.||Bacterial DNA Replication II||Ch8|
|4||Fri.||Eukaryotic DNA Replication I||Ch8|
|5||Tues.||Eukaryotic DNA Replication II||7: 157-174; 6: 117-126|
|6||Fri.||Recombinant DNA I||19: 661-662; 21: 739-764; 22: 783-794|
|Tues.||Exam I (Covers Lectures 1-6) See exam rules below|
|7||Fri.||Recombinant DNA II|
|8||Tues.||DNA Mutability||1: pp 15-17; 6: pp 102-107; 8, pp 198-209; 9, pp. 258-260.|
|9||Fri.||DNA Repair||9: pp 257-281|
|10||Tues.||DNA Recombination||1: 6-13; 9: 275-278; 10: 283-310; 10: 314-317; 11: 365-369.|
|11||Fri.||DNA Transposition||11: 319-321; 11: 331-359; 11: 362-365.|
|Fri||Exam II (Covers Lectures 7-12)|
|13||Tues.||Transcriptional Regulation in Bacteria||16: 542-587 5: 82-94|
|14||Fri||Transcriptional Regulation in Bacteria||16: 542-587 18: 633-640|
|15||Tues.||Transcription in Eukaryotes||7: 157-187 12: 396-414|
|16||Fri.||Transcriptional Regulation in Eukaryotes||17:589-632|
|17||Tues.||Transcriptional Regulation in Eukaryotes||17: 589-632|
|18||Fri.||Transcriptional Regulation in Eukaryotes|
|Tues.||Exam III (Covers Lectures 13-18)|
|20||Tues||Post-transcriptional Control: Splicing & Polyadenylation (cont.)||13, 18‡|
|21||Fri.||Post-transcriptional Control: Splicing & Polyadenylation (cont.)||13, 18‡|
|22||Tues.||RNA World, Ribozymes, RNA Splicing||13, 18‡|
|23||Fri.||Control of mRNA Translation, Stability, Localization||14|
|24||Tues.||Translation: Bacterial & Eukaryotic||14, 15 ‡|
|25||Fri||Translation: Bacterial & Eukaryotic|
|Exam IV (Covers Lectures 19-25)|
Course Satisfies Departmental Learning Goals
1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research and that is required for post-graduate exams and studies.
2. Students should demonstrate the ability to find and evaluate information about specific biological systems or problems.
3. Students should demonstrate the ability to design experiments and critically analyze data.
Exams, Assignments, and Grading Policy
Hourly examinations will be given at the end of each series of lectures on the indicated dates; the first three will be held during regular class hours and students will sit in assigned seats in the SERC 111 classroom or in an "overflow" room that will be announced. The fourth hourly examination will be held during the final exam period, in a room to be announced. There will be no cumulative final exam and the graded final exam will not be returned to students.
All exams are closed book. Students are expected to follow the Rutgers Academic Integrity Code and will not be permitted to have bags, phones or papers by their seats. Students caught cheating will get a zero for the exam and the Dean's office will be notified. All four examinations will be averaged and weighted equally in assigning final grades. In the rare case that an exam question has not been clearly graded, a request for re-grading will be considered WITHIN ONE WEEK AFTER EXAM PAPERS HAVE BEEN RETURNED. Note that the entire exam will be regraded so that points may be added or subtracted due to grading errors.
Students who are unable to take an hourly exam at the scheduled time because of compelling reasons supported by documentation, such as a note from their physician - NOT THE HEALTH CENTER - may be permitted to take a make-up examination. However, a 5 pt deduction will be made for the first make-up, 10 pts for the second and 20 pts for the third. Arrangements should be made with Prof. Brill.
5% of the final grade will be determine by in class iClicker responses
Watson, J.D., et al. (2008) Molecular Biology of the Gene, 6th Ed., Pearson. (ISBN: 9780805395921) (preferred)
Watson, J.D., et al. (2014) Molecular Biology of the Gene, 7th Ed., Pearson. (ISBN: 9780321762436)
Note: Either edition is acceptable. The 7th Ed contains all the information within the 6th Ed, plus some new chapters and figures that will not be covered in this class.
‡Supplemental Readings will be posted on the Sakai sites for the course.
Students are required to purchase and register an iClicker for this course. They are available at the Rutgers Bookstore.
phone: (732) 235-4197
phone: (732) 235-5333
Nelson Biology Labs, A-322
phone: (732) 445-1016
** All information is subject to change at the discretion of the course coordinator.