01:146:475 Laboratory in Immunology
This course may be used to fulfill the elective and laboratory requirements of the Biological Sciences major.
Prerequisite: Students are required to take one of the following courses before taking this course: Immunology 01:146:474, Organic Chemistry 160:307-308, Genetics 447:380, Molecular Biology 146:478, Molecular Biology and Biochemistry 694:408, Human Histology 146:322, Advanced Neurobiology 146:445, or Advanced Cell Biology 146:470
The overall objective of this course is to give the advanced biology students experience with addressing immunological questions through experimentation. A representative experiment will be the analysis of different lymphocyte subsets in distinct lymphoid tissues of the mouse. Specific techniques will analyze RNA and protein expression in different immune cell populations and explore the functions of these unique cells
Representative Syllabus (may be slightly modified year to year):
Title of Lab
1.Introduction to immunology lab and examination of peripheral blood smears
2. Isolation of mouse spleen mononuclear cells by density gradient centrifugation
3. Identification of B cell and T cell populations of mouse spleen and thymus by flow cytometry
4. Collection of mouse peritoneal lavage and harvest of mouse lymphoid tissues
5. Measurement of inflammatory cytokines secreted from activated macrophages by ELISA
6. Preparation of RNA from mouse lymphoid tissues and analysis of CD4 expression by RT-PCR
7. Activation of human B cells and preparation of proteins from naïve and activated B cells
8. Quantification of a B cell-specific protein by Western blot analysis
Sakai website will be active during the semester
Course Satisfies Learning Goals
1. Master factual and conceptual knowledge in Immunology that will provide a solid foundation for success in advanced training and professional careers.
2. Develop an ability to summarize, integrate and organize information and relate it to disease outcomes
3. Use scientific reasoning to evaluate the potential for current research and new discoveries to improve our understanding of immunology and its relevance to human health and to our society.
4. Use experimental techniques to address immunological questions.
Exams, Assignments, and Grading Policy
Grades are weighted most heavily on four lab reports and/or presentation. Attendance and participation in the labs are also factors in the final grade.
If this course is closed, please use the following link to add your name to the wait list: Wait List Sign Up for Spring 2020 Courses . If you have any questions, please contact the Division of Life Sciences - Office of Undergraduate Instruction at 848-445-2075 or visit our office at Nelson Biological Laboratories B112, Busch Campus.
B336 Nelson Labs
Other Faculty Participants:
Dr. Brian Daniels
Nelson Labs B314
Nelson Labs D411
Nelson Labs B303
** All information is subject to change at the discretion of the course coordinator.