01:146:471 Advanced Cell Biology Laboratory
This course may be used to fulfill the elective and laboratory requirements of the Biological Sciences major.
Advanced Cell Biology 01:146:470 and 01:119:117 or 01:119:102, or permission of the instructor.
Course objective: To provide participants with hands-on experience in selected techniques of molecular cell biology, with special emphasis on experimental design and communication skills.
The lab will have three modules:
(1) Cell biology of prokaryotes
(2) Cell biology of eukaryotes
(3) Guided research projects.
The jellyfish green fluorescent protein (GFP) gene and variants will be used as a unifying theme. Tentative schedules are listed below.
Module 1: Bacterial response to environment
Week 1, introduction to lab safety and to GFP, culture of bacteria, transformation of bacteria with GFP plasmids
Week 2, isolation of plasmid DNA, restriction digest and electrophoretic analysis of DNA
Week 3, fluorescence microscopy of GFP-tagged bacteria, response of bacteria to environmental stimuli
Week 4, oral and written presentation of data from module 1
Module 2: Diversity of mammalian cell types
Week 1, introduction to mammalian cell culture, DNA transfection of mammalian cells with organelle-targeted GFP plasmids
Week 2, demonstration of fluorescence activated cell sorting, enzyme linked immunosorbent assay of GFP
Week 3, fractionation of organelles via the virtual centrifugation lab, polyacrylamide gel electrophoresis and western blotting of GFP
Week 4, oral and written presentation of data from module 2
Module 3: Cellular dynamics
Week 1, internet tools for molecular cell biology
Week 2, polymerase chain reaction, research project design, selection and consultation with mentors
Weeks 3 to 5, project experiments (students may need to spend additional time in the lab depending on the type of experiment to be performed)
Week 6, oral and written presentation of data from module 3
Course Satisfies Learning Goals
Basic experimental techniques; integration and organization of data; scientific reasoning and communication
Exams, Assignments, and Grading Policy
Take-home assignments; lab procedure write-ups; oral presentations
If this course is closed, please use the following link to add your name to the wait list: Wait List Sign Up for Spring 2020 Courses . If you have any questions, please contact the Division of Life Sciences - Office of Undergraduate Instruction at 848-445-2075 or visit our office at Nelson Biological Laboratories B112, Busch Campus.
** All information is subject to change at the discretion of the course coordinator.