• Course objective: To provide participants with hands-on experience in selected techniques of molecular cell biology, with special emphasis on experimental design and communication skills.
  • Semester Offered: Spring
  • Credits: 3
  • Course URL: Canvas

Prerequisites 

Advanced Cell Biology 01:146:470 and 01:119:117 or 01:119:102, or permission of the instructor.

Course Description 

Course objective: To provide participants with hands-on experience in selected techniques of molecular cell biology, with special emphasis on experimental design and communication skills.

The lab will have three modules:

  1. Cell biology of prokaryotes
  2. Cell biology of eukaryotes
  3. Guided research projects

The jellyfish green fluorescent protein (GFP) gene and variants will be used as a unifying theme. Tentative schedules are listed below.

Module 1: Bacterial response to environment 

  • Week 1: Introduction to lab safety and to GFP, culture of bacteria, transformation of bacteria with GFP plasmids
  • Week 2: Isolation of plasmid DNA, restriction digest and electrophoretic analysis of DNA
  • Week 3: Fluorescence microscopy of GFP-tagged bacteria, response of bacteria to environmental stimuli
  • Week 4: Oral and written presentation of data from module 1

Module 2: Diversity of mammalian cell types 

  • Week 1: Introduction to mammalian cell culture, DNA transfection of mammalian cells with organelle-targeted GFP plasmids
  • Week 2: Demonstration of fluorescence activated cell sorting, enzyme linked immunosorbent assay of GFP
  • Week 3: Fractionation of organelles via the virtual centrifugation lab, polyacrylamide gel electrophoresis and western blotting of GFP
  • Week 4: Oral and written presentation of data from module 2

Module 3: Cellular dynamics 

  • Week 1: Internet tools for molecular cell biology
  • Week 2: Polymerase chain reaction, research project design, selection and consultation with mentors
  • Weeks 3-5: Project experiments (students may need to spend additional time in the lab depending on the type of experiment to be performed)
  • Week 6: Oral and written presentation of data from module 3

Course URL

Canvas

Course Satisfies Learning Goals 

Basic experimental techniques; integration and organization of data; scientific reasoning and communication

Exams, Assignments, and Grading Policy 

Take-home assignments; lab procedure write-ups; oral presentations

Course Materials 

TBA

Course Closed?

If this course is closed, please use the following link to add your name to the wait list: Wait List Sign Up . If you have any questions, please contact the Division of Life Sciences - Office of Undergraduate Instruction at 848-445-2075 or visit our office at Nelson Biological Laboratories B112, Busch Campus.

Faculty 

Course meets Tu & Th 1-4 PM, Room Nelson B417

Dunne Fong
B424 Nelson
(848) 445 5268
This email address is being protected from spambots. You need JavaScript enabled to view it. 


** All information is subject to change at the discretion of the course coordinator.