Prerequisites: Organic Chemistry (01:160:307) and Biochemistry (01:694:301, 11:115:301, or 01:694:407)
Course Description: This course provides familiarization with protein and enzyme techniques in the laboratory, and properties of amino acids and sugars. There is some material dealing with DNA and very basic molecular biology. The lab complements the material in the course, 694:301, but is not taught in parallel with 301.
Course satisfies Learning Goals
1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research and that is required for post-graduate exams and studies.
3. Students should demonstrate the ability to design experiments and critically analyze data.
Lab 1 Course Introduction, Pipeting Exercises
Lab 2 DNA Concentrations, Plate Cells
Lab 3 Photometry, Set up Bacterial ON Cultures
Lab 4 Plasmid Purification
Lab 5 PCR/ Restriction Enzymes
Lab 6 DNA Gel Electrophoresis
Lab 7 Bioinformatics - DNA Sequence Analysis
Lab 8 Induce protein expression
Lab 9 Chromatography Nickel Column
Lab 10 Protein Concentration Assay
Lab 11 Protein Gel Electrophoresis
Lab 12 Galactosidase Km, Galactosidase Inhibitio
Lab 13 Protein Structure Analysis
Assignments and Grading Policy: Grade is based on lab reports (50%), quizzes (50%). A laboratory course can't be taken in absentia, you must show up and participate in running the experiments.
Required text: Online notes posted on Canvas
Course Closed? There is only limited space in the laboratory so No special permission numbers will be administered for this course. If the course is closed you should continue to check WebReg to see if a spot opens.
Dr. Gabriel Villegas
** All information is subject to change at the discretion of the course coordinator.