Prerequisites
Advanced Neurobiology I 01:146:445 and 01:119:117 or 01:119:102
Course Description
Advanced laboratory methods in Neurobiology. Electrophysiological and immunohistochemical techniques will be used to explore the functional and structural features of nerve cells. The course consists of three multi-week projects that focus on voltage-gated potassium channels in N2a cells and the whole-cell ionic currents in hippocampal neurons. The laboratory environment is open and students are encouraged to work independently.
Techniques covered:
- Microelectrode fabrication
- Use of inverted microscopes and micromanipulators
- Use of computer-controlled data acquisition hardware
- Single-channel voltage-clamp
- Whole-cell voltage-clamp
- Whole-cell current clamp
Course URL
Sakai
Course Satisfies Learning Goals
1. Master factual and conceptual knowledge in neuroscience that will provide a solid foundation for success in advanced training and professional careers.
2. Develop an ability to summarize, integrate and organize information.
3. Use scientific reasoning to evaluate the potential for current research and new discoveries to improve our understanding of the nervous system and its relevance to human health and to our society.
Exams, Assignments, and Grading Policy
Three laboratory reports (one from each of the projects) as well as participation in class meetings and discussions
Course Materials
Supplied protocols and assigned papers
Course Closed?
Enrollment is by special permission only. Please contact the course instructor for more information.
Faculty
Dr. Mark Plummer
** All information is subject to change at the discretion of the course coordinator.
