INTRODUCTORY BIOCHEMISTRY LABORATORY (01:694:313)
NOT for students majoring in Molecular Biology and Biochemistry.
This course may be used to fulfill the elective requirements and laboratory requirement of the Biological Sciences major.
Offered: Fall, Spring, Summer
Prerequisites: Organic Chemistry (01:160:307) and Biochemistry (01:694:301, 11:115:301, or 01:694:407)
Course Description: This course provides familiarization with protein and enzyme techniques in the laboratory, and properties of amino acids and sugars. There is some material dealing with DNA and very basic molecular biology. The lab complements the material in the course, 694:301, but is not taught in parallel with 301.
Course satisfies Learning Goals
1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research and that is required for post-graduate exams and studies.
3. Students should demonstrate the ability to design experiments and critically analyze data.
Lab 1 Course Introduction, Pipeting Exercises
Lab 2 DNA Concentrations, Plate Cells
Lab 3 Photometry, Set up Bacterial ON Cultures
Lab 4 Plasmid Purification
Lab 5 PCR/ Restriction Enzymes
Lab 6 DNA Gel Electrophoresis
Lab 7 Bioinformatics - DNA Sequence Analysis
Common Mid-Term Exam No labs
Lab 8 Induce protein expression
Lab 9 Chromatography Nickel Column
Lab 10 Protein Concentration Assay
Lab 11 Protein Gel Electrophoresis
Lab 12 Galactosidase Km, Galactosidase Inhibition
Lab 13 Protein Structure Analysis
Common Final Exam
Exams, Assignments, and Grading Policy: Grade is based on exams (50%), lab reports (40%), performance in lab, and attendance (10%). A laboratory course can't be taken in absentia, you must show up and participate in running the experiments.
Required text: Online notes posted on Sakai
Course Closed? There is only limited space in the laboratory so No special permission numbers will be administered for this course. If the course is closed you should continue to check WebReg to see if a spot opens.
** All information is subject to change at the discretion of the course coordinator.